An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysisA high-throughput approach, combining deep mutational scanning and structure-informed design, generated miniature AsCas12f variants with enhanced activity, enabling efficient knock-in/knock-out activities and transcriptional activation in mice using an all-in-one AAV vector.A high-throughput approach, combining deep mutational scanning and structure-informed design, generated miniature AsCas12f variants with enhanced activity, enabling efficient knock-in/knock-out activities and transcriptional activation in mice using an all-in-one AAV vector.Tomohiro Hino, Satoshi N. Omura, Ryoya Nakagawa, Tomoki Togashi, Satoru N. Takeda, Takafumi Hiramoto, Satoshi Tasaka, Hisato Hirano, Takeshi Tokuyama, Hideki Uosaki, Soh Ishiguro, Madina Kagieva, Hiroyuki Yamano, Yuki Ozaki, Daisuke Motooka, Hideto Mori, Yuhei Kirita, Yoshiaki Kise, Yuzuru Itoh, Satoaki Matoba, Hiroyuki Aburatani, Nozomu Yachie, Tautvydas Karvelis, Virginijus Siksnys, Tsukasa Ohmori, Atsushi Hoshino, Osamu Nurekihttps://www.cell.com/cell/fulltext/S0092-8674(23)00963-7?rss=yeshttp://www.cell.com/cell/inpress.rssCellCell RSS feed.Wireless News CampaignSeptember 30, 2023

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