Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease ActivityThe cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.The cryo-EM structures of Cas12a and smFRET reveal the activation mechanism for target DNA cleavage and indiscriminate ssDNA degradation. The displacement of the R-loop by crRNA suggests a mechanism to stop unspecific ssDNA degradation, resetting the endonuclease to target a new DNA sequence.Stefano Stella, Pablo Mesa, Johannes Thomsen, Bijoya Paul, Pablo Alcón, Simon B. Jensen, Bhargav Saligram, Matias E. Moses, Nikos S. Hatzakis, Guillermo Montoyahttps://secure.jbs.elsevierhealth.com/action/getSharedSiteSession?redirect=https%3A%2F%2Fwww.cell.com%2Fcell%2Ffulltext%2FS0092-8674%2818%2931401-6%3Frss%3Dyes&rc=0http://www.cell.com/cell/inpress.rssCellCell RSS feed.Wireless News CampaignNovember 30, 2018

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