Principles of cotranslational mitochondrial protein importSelective ribosome profiling reveals that nearly 20% of mitochondrial proteins in human cells are imported during translation on cytosolic ribosomes. Cotranslational import requires an N-terminal presequence but is delayed until a large domain emerges and prioritizes large multi-domain proteins. This multi-layered sorting mechanism enables cells to balance the import demands for the mitochondrial proteome with diverse biophysical properties.Selective ribosome profiling reveals that nearly 20% of mitochondrial proteins in human cells are imported during translation on cytosolic ribosomes. Cotranslational import requires an N-terminal presequence but is delayed until a large domain emerges and prioritizes large multi-domain proteins. This multi-layered sorting mechanism enables cells to balance the import demands for the mitochondrial proteome with diverse biophysical properties.Zikun Zhu, Saurav Mallik, Taylor A. Stevens, Riming Huang, Emmanuel D. Levy, Shu-ou Shanhttps://www.cell.com/cell/fulltext/S0092-8674(25)00811-6?rss=yeshttp://www.cell.com/cell/inpress.rssCellCell RSS feed.Wireless News CampaignAugust 12, 2025

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